Mitosis is a replicative cell division necessary for cell growth, regeneration and replacement, wherein the dividing cell segregates all of its chromosomes equally into two daughter cells. Concomitantly, the cytoplasmic components are also segregated through cytokinesis. Microtubules play several important roles during mitosis. They are organized to form the mitotic spindle, necessary for segregation of daughter chromosomes. In addition, microtubules and the mitotic spindle direct the placement and assembly of the cleavage furrow during cytokinesis. Thus, misregulation of mitotic spindle stability or dynamics can lead to improper chromosome segregation, potentially leading to cell death or cancer progression. During mitosis, microtubules are nucleated from a variety of sources in the cell. The nucleation and dynamics of three types of microtubules that emanate from the centrosome (kinetochore, polar and astral) are regulated by a large number of proteins. Among these are several microtubule-associated proteins, which bind to microtubules and can regulate their stability.
The GTSE1 (G2 and S-phase expressed 1) protein was recently discovered to be a microtubule-associated protein. In interphase, GTSE1 interacts directly with the microtubule lattice, and also tracks the growing ends of microtubules. The latter activity has been shown to regulate cell migration. GTSE1 is found overexpressed in several tumors, and preliminary results show that GTSE1 is also required for progression through mitosis, although the processes affected are unclear. I aim to elucidate the molecular mechanism by which GTSE1 controls mitotic spindle assembly and cytokinesis in mammalian cells.
The aims of this project are:
• To investigate the role played by GTSE1 in controlling microtubule stability and dynamics during mitosis
• To define the pathways by which GTSE1 functions during mitosis, especially its role in spindle formation and cytokinesis
• Studying the biochemistry of GTSE1 protein
Shweta Anil Bendre
Max Planck Institute for Molecular Physiology,
Otto-Hahn Strasse 11,
44227 Dortmund, Germany.
Phone: +49 (231) 133 2106
Max Planck Institute for Molecular Physiology, Dortmund, Germany, PhD student,
University of Bremen, Germany, Master in Biochemistry and Molecular Biology,
October 2010-February 2013
University of Pune, India, Bachelor in Microbiology, July 2007-June 2010
International Max Planck Research School (IMPRS) in Chemical Biology PhD fellowship, June 2013-present
Second rank at the Poster Presentation of the BMB Symposium, February 2012, University of Bremen, Germany
University topper for Bachelor of Microbiology, University of Pune, India
Late Dr. Ramchandra Joshi memorial award for ‘Securing highest marks at B.Sc. - Microbiology’
Jaydeep Naik memorial talent of the year award for ‘All-round performance at the Third Year B.Sc. - Microbiology’
Late Jaydeep Naik memorial award for ‘Talent of the year-First Year and Second Year 1st Class Microbiology’